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KMID : 0380620120440060763
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Korean Journal of Food Science and Technology
2012 Volume.44 No. 6 p.763 ~ p.771
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Antioxidant Activity of Different Parts of Lespedeza bicolor and Isolation of Antioxidant Compound
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Lee Jae-Hak
Jhoo Jin-Woo
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Abstract
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In this study, total antioxidant properties of extracts from different parts of Lespedeza bicolor were determined using techniques of measuring 1,1-diphenyl-2-picryl hydrazyl/2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)-radical scavenging activity and total phenolic contents. The total antioxidant activities of leaf, stem and root extracts from various solvents (water, 50, 70, 100% ethanol, and hot-water) indicated that 50 and 70% ethanol extracts have high radical scavenging activities and phenolic contents. A systematic approach was used to determine the total antioxidant activity of different solvent fractions of the Lespedeza bicolor extracts, partitioning with chloroform, ethyl acetate, n-butanol, and water, and the ethyl acetate fraction was found to have the strongest antioxidant activity. Antioxidant assay-guided isolation was carried out to isolate potential antioxidant compounds. The ethyl acetate fraction of the leaf extract was subjected to silica gel, LH-20 and RP-18 column chromatography successively, and afforded compound 1, which was identified as eriodictyol by NMR and MS analysis, after which its antioxidant activity was determined.
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KEYWORD
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Lespedeza bicolor, antioxidant activity, radical scavenging activity, flavonoid, eriodictyol
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